Introduction In today's lab exercise, we will examine one of the factors that influence enzyme activity. All enzymes are proteins. The function of enzymes is to accelerate defined chemical reactions by alternating the rate of the reaction. They will not trigger a reaction that would not occur naturally. By having a particular enzyme to catalyze each of the chemical reactions that occur in a living cell, an organism can maintain total control of its metabolism. Catalase is an enzyme found in cell organelles called peroxisomes. Catalyzes the degradation of hydrogen peroxide (which is toxic to the cell) into oxygen and water Catalase2H2O2  O2 + 2H2OH Hydrogen peroxide (enzyme) Oxygen Water (substrate) (Products) In a reaction catalysed by an enzyme, the compound on which the enzyme acts is called its substrate and the results of the reaction are called products. Enzymes are said to be specific, that is, they are only found on certain substrates. In the above reaction, hydrogen peroxide is the substrate, catalase is the enzyme, and oxygen and water are the products. If the reaction is ongoing, the released oxygen will cause bubbles to form. The enzyme emerges from the reaction unchanged and can be used over and over again. One way to get more product in a reaction is to add more substrate. Another way is to add more enzyme. A limit will be reached depending on the amount of substrate present. Adding more enzyme after the substrate has been completely converted is a useless action since the enzyme is not used in the reaction as the substrate is. In this experiment, the optimal temperature for catalase activation... in the middle of the paper. .....hol; and at the fourth, 3 ml of glucose solution. Gently shake each tube and note the reaction. Record the results.EFFECT OF TEMPERATURE ON ENZYMES1. Place 3 ml of solution 1 in a test tube and place it in a glass of ice. Cool the solution to approximately 0ºC. Leaving the tube on ice, add 3 ml of H2O2 to the tube. Note the immediate result and when the solution begins to warm and record the result. Perform the same test using solution 1 at room temperature and record the result. Perform the same test using solution 1 at 37ºC by placing 3 ml of solution 1 in a test tube and placing it in a water bath at 37ºC. Take care that the temperature does not exceed 37ºC. Record the result. Place 3 ml of solution 1 in a test tube. Heat it to boiling in a water bath. Let it cool and then add 3 ml of H2O2 to the test tube. Write down the result and record it.
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