Mushrooms are creatures unique to the living world and cannot be classified as either plants or animals. It has become one of the human food sources. There has recently been interest in mushrooms not only as a healthy protein-rich vegetable, but also as an important source of biologically active compounds of medical value. Based on Ooi & Liu (2000), schizophyllan from S. commune and lentinan, an interferon-stimulating polysaccharide produced by L. edodes, are products that have been isolated from fungi to produce anticancer drugs. Nowadays, mushroom cultivation has become an agronomy-generating activity that can be applied to the small industrial farmer. Therefore, to grow the mushroom there are several stages known as medium culture preparation, egg preparation, medium substrate preparation, egg laying, egg race, fruiting and harvesting (refer to Figure 1 in Appendix 1). The first steps in mushroom cultivation are preparation of the culture media. Mushroom mycelium needs nutrition to grow. Agar (seaweed) contains almost no nutrients, but acts as a gelling agent when mixed with water, so that the mycelium has a flat, solid surface to grow on. A combination of agar, water and nutrients provides a satisfactory method for growing healthy mycelium (Ogden & Prowse, 2004). The mycelium growing on the soil surface will later be used to inoculate larger quantities of substrates such as grain. Test tubes or Petri dishes used as culture containers. Young and vigorous mycelium obtained from a young fruiting body of a mushroom. The mycelium should be white and growing from the tissue. If yellow, blue, green or gray mycelia form elsewhere on the surface, they are fungal contaminants. A cre...... middle of the document ......ots=psZWcIr-RN&sig=89w1Eyy-xVf_k45DbIqbtyKrbYE#v=onepage&q&f=falseOgden, A. & Prowse, K. (2004). How to spawn oyster mushroom grits the easy way. In R. Gush (Ed.), Mushroom Growers' Handbook 1 (pp. 62-74). Seoul, Korea: Mush World. Retrieved from http://www.fungifun.org/mushworld/Oyster-Mushroom-Cultivation/mushroom-growers-handbook-1-mushworld-com-chapter-4-1.pdfOoi, V.E.C., & Liu, F. (2000) . Immunomodulation and anti-tumor activity of polysaccharide-protein complexes. Current Medicinal Chemistry, 7(7), 715-729. Retrieved from http://www.ingentaconnect.com/content/ben/cmc/2000/00000007/00000007/art00004Shah, Z. A., Ashraf, M., & Ishtiaq, C. M. (2004). Comparative study on the cultivation and production performance of the pleurotus mushroom (Pleurotus ostreatus) on different substrates (wheat straw, leaves, sawdust). Pakistan Journal of Nutrition, 3(3), 158-160.