The effect of temperature on beet membranes Purpose of the research: The purpose of this investigation is to determine what type of effect increasing temperature will have on the plasma membrane of a cell of beetroot. IntroductionBeetroot contains a red pigment that is retained in cells by membranes. If the membranes are damaged, the pigment “betalain” will leak out. The amount of pigment that escapes can be assessed, as the “betalain” will color the water surrounding the cell. If the water with the beetroot slices is heated to different temperatures, will the temperature have any effect on the color of the solution? HypothesisI think more red pigment will be released as the temperature increases. When beetroot slices in water are heated, the high temperature will provide more kinetic energy. The increased energy will cause the molecules inside the beetroot slices to move faster. The fast movement will likely cause damage and break the cell membrane allowing the red pigment to fade from the cells.Temperature (C°) Color of beetroot water Color intensity (arbitrary units)0 Pale cream color 0.0220 Darker cream color 0. 0240 Pink with a red mix 0.0660 dark red 1.0080 Orange red .65100 Yellow/orange .14Table 1: Observations on the colors of beet water from my data (group 5)Temperature(C° ± 5.0 C°) Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Mean SD0 0.03 0.01 0.13 0.05 0.02 0.00 0.01 0.04 0.0420 0.04 0.01 0.06 0, 00 0.02 0, 01 0.00 0.02 0.0240 0.09 0.05 0.06 0.15 0.06 0.00 0.03 0.06 0.0460 1.45 0.56 0 .90 0.84 1 .00 0.05 0.59 0.77 0.4080 1.45 0.62 2.00 0.65 0.65 0.74 0.87 1.00 0.49100... ... half of the paper ......some groups had found several leaks of the pigment in the test tubes with water. You could improve by being extra careful when cutting the beetroot, however using a different type of equipment instead of the knife should make the cut more accurate. The water baths were well controlled and thermometers helped control desired temperatures. I think the water baths were accurate enough, but having two thermometers in each bath perhaps would have helped keep the temperature readings more accurate. We were not given any instructions to shake or not shake the tubes with the colored solutions before inserting them into the spectrophotometer to read the absorbance. Shaking each test tube a number of times before inserting it into the spectrophotometer could improve the accuracy of the absorbance of the solutions.