Topic > Peroxidase Assay - 1096

When analyzed, the data showed that the enzyme had maximum activity at pH 5 by a wide margin. The other pH balances did not come close to this value. The pH with the lowest enzyme activity was pH 9 with the value of 0.00005. pH 3 and 7 differed by 0.000133 in enzymatic activity, while pH 7 was more functional. These data imply that the optimal pH balance for peroxidase was 5; any higher or lower pH balances, such as those tested, denature the enzyme or have a slower than optimal reaction rate. Although the optimal pH for peroxidase was pH 5, most enzymes work best at pH levels between six and eight (Biology Certificate). For example, the enzyme lactase, responsible for breaking down lactose, has an optimal pH of 6 (Siskin). At the other end of the spectrum, pepsin, found in stomach acid, has an optimal value that works best between 1.5 and 1.6 pH (Worthington Biochemical Corporation). The peroxidase enzyme is found between the two enzymes, lactase and pepsin; all three of these enzymes have an optimal pH which is found in the acidic part of the